See Also: NRC Membership Listing > MultiPhoton Laser Scanning Microscope (MPLSM)
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A microscopic research technique to visualize fluorescent molecules
in living cells.
The Neuroscience
Research Center recently acquired a MultiPhoton Laser Scanning Microscope
(MPLSM), an instrument that represents the latest development in
imaging technology.
Traditional
microscopes use regular light, which can cause tissue samples to
appear blurry. Confocal microscopes eliminate much of the blurriness
by blocking out light emitted from structures not in focus, producing
a sharper image. Living tissue specimens, however, can be damaged
by confocal microscopy.
The multiphoton
confocal microscope is superior to traditional and confocal microscopes,
in that it uses infrared light to illuminate only a small dot of
tissue at a time. Moreover, in living tissue, damage is minimized.
Because the light beam penetrates deeply, a greater volume of tissue
can be examined.
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